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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
02/06/2000 |
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Data da última atualização: |
02/06/2000 |
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Autoria: |
MAKITA, M.; ALBRECHT, J. C. |
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Título: |
Utilizacao do metodo hematoxilina na identificacao de genotipos de trigo tolerantes ao aluminio. |
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Ano de publicação: |
1992 |
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Fonte/Imprenta: |
In: SEMINARIO SOBRE OS PROGRESSOS DA PESQUISA AGRONOMICA NA REGIAO DOS CERRADOS, 1991, Cuiaba. [Anais]. Brasilia: EMBRAPA-CPAC/EMPA/JICA, 1992. |
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Páginas: |
p.35-41. |
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Idioma: |
Português |
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Conteúdo: |
Como o aluminio contido no solo prejudica na multiplicacao e crescimento das raizes do trigo, e utilizado o metodo de investigacao de tolerancia a toxidez do aluminio atraves do desenvolvimento das raizes. Porem, o novo metodo e feito atraves da coloracao das raizes com o uso de hematoxilina. Ao ser comparado com metodo anterior, verificamos que e o seu merito o de ser simples no manuseio e possuir alta repetividade. 1) O metodo da coloracao pela hematoxilina para a identificacao da tolerancia ao aluminio e de utilidade pratica. Pode ser usado para a triagem de grande numero de plantas, em um programa de melhororamento para preferencia ao aluminio em estudos de sua genetica. 2). As produtividades de variedades tolerantes ao aluminio, no cultivo da estacao chuvosa, foram menores do que as obtidas no periodo da seca para variedades nao tolerantes. Portanto, de acordo com os resultados deste experimento, o nivel de tolerancia necessaria atingiu o indice tres (Tolerantes) e nao foram encontradas variedades com indice um (Altamente Tolerantes). 3). O germoplasma brasileiro de trigo apresenta variedades tolerantes, como foi citado anteriomente. Todas as variedades de destaque com elevada tolerancia contem genes de ancestrais obtidos no Brasil. Pode-se afirmar que as variedades brasileiras sao importantes fontes de melhoramento na tolerancia ao aluminio. |
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Palavras-Chave: |
Aluminium; Hematoxilina; Hematoxylin; Wheats. |
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Thesagro: |
Alumínio; Cerrado; Genótipo; Trigo; Triticum Aestivum. |
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Categoria do assunto: |
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Marc: |
LEADER 02124naa a2200253 a 4500
001 1555240
005 2000-06-02
008 1992 bl uuuu u00u1 u #d
100 1 $aMAKITA, M.
245 $aUtilizacao do metodo hematoxilina na identificacao de genotipos de trigo tolerantes ao aluminio.
260 $c1992
300 $ap.35-41.
520 $aComo o aluminio contido no solo prejudica na multiplicacao e crescimento das raizes do trigo, e utilizado o metodo de investigacao de tolerancia a toxidez do aluminio atraves do desenvolvimento das raizes. Porem, o novo metodo e feito atraves da coloracao das raizes com o uso de hematoxilina. Ao ser comparado com metodo anterior, verificamos que e o seu merito o de ser simples no manuseio e possuir alta repetividade. 1) O metodo da coloracao pela hematoxilina para a identificacao da tolerancia ao aluminio e de utilidade pratica. Pode ser usado para a triagem de grande numero de plantas, em um programa de melhororamento para preferencia ao aluminio em estudos de sua genetica. 2). As produtividades de variedades tolerantes ao aluminio, no cultivo da estacao chuvosa, foram menores do que as obtidas no periodo da seca para variedades nao tolerantes. Portanto, de acordo com os resultados deste experimento, o nivel de tolerancia necessaria atingiu o indice tres (Tolerantes) e nao foram encontradas variedades com indice um (Altamente Tolerantes). 3). O germoplasma brasileiro de trigo apresenta variedades tolerantes, como foi citado anteriomente. Todas as variedades de destaque com elevada tolerancia contem genes de ancestrais obtidos no Brasil. Pode-se afirmar que as variedades brasileiras sao importantes fontes de melhoramento na tolerancia ao aluminio.
650 $aAlumínio
650 $aCerrado
650 $aGenótipo
650 $aTrigo
650 $aTriticum Aestivum
653 $aAluminium
653 $aHematoxilina
653 $aHematoxylin
653 $aWheats
700 1 $aALBRECHT, J. C.
773 $tIn: SEMINARIO SOBRE OS PROGRESSOS DA PESQUISA AGRONOMICA NA REGIAO DOS CERRADOS, 1991, Cuiaba. [Anais]. Brasilia: EMBRAPA-CPAC/EMPA/JICA, 1992.
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Registro original: |
Embrapa Cerrados (CPAC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agrossilvipastoril. |
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Data corrente: |
18/02/2019 |
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Data da última atualização: |
18/02/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
LIMA, L. R.; MOMBACH, M. A.; PEDREIRA, B. C. e; FERREIRA, D. C.; CARVALHO, P. de; AMORIM, K. R. R. de; WEIMER, P. J.; CABRAL, L. da S. |
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Afiliação: |
LENI RODRIGUES LIMA, UFMT, CUIABA, MT; MIRCÉIA ANGELE MOMBACH, UFMT, CUIABA, MT; BRUNO CARNEIRO E PEDREIRA, CPAMT; DANIELA CRISTINA FERREIRA, UFMT, CUIABA, MT; PERIVALDO DE CARVALHO, UFMT, CUIABA, MT; KARITHA REGIANE RIBEIRO DE AMORIM, UFMT, CUIABA, MT; PAUL JAMES WEIMER, UNIVERSITY OF WISCONSIN, MADISON, US; LUCIANO DA SILVA CABRAL, UFMT, CUIABA, MT. |
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Título: |
Methanogens and rumen protozoa in cattle during transition to high grain diets. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 55.; CONGRESSO BRASILEIRO DE ZOOTECNIA, 28., 2018, Goiânia. Construindo saberes, formando pessoas e transformando a produção animal: anais. Viçosa: Sociedade Brasileira de Zootecnia; Brasília, DF: Associação Brasileira de Zootecnistas. |
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Páginas: |
não paginado. |
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Idioma: |
Inglês |
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Conteúdo: |
The rumen is a very complex ecosystem in which there is a high diversity and concentration of rumen microbes. Animals, microbial and diets effects shape the rumen microbial population what make the rumen microbial manipulation a hard task to be done. There are many aspects about rumen microbial what the scientists and nutritionists would like to change, but the reduction in rumen methanogens and its activities is probably the first one. Considering the probable association between protozoa and rumen methanogens, this study aimed to evaluate their populations in the rumen of beef cattle during transition from a forage to high grains diets by qPCR. Four rumen fitted male Nellore Cattle were used, which were submit to diets from Brachiaria brizantha grass to high grains, from which rumen samples (solid and fluid) were collected when the diets were composed by zero (grazing animals), 50, 60, 70, 80 and 90% of concentrate on dry matter basis, as well as to get pH measurements. The DNA of rumen microbial population was isolated by using beads, phenol and chloroform, which makes possible to get high quantity and quality of DNA isolated. The DNA of rumen microbial population was amplified using groups primers (protozoa and methanogens) by qPCR method, which permits to calculate the genes copies numbers for every group. The rumen pH ranged from 6.51 to 5.73 for forage fed animals and for 90% concentrate fed animals, respectively. We found that protozoa numbers (log10 genes copies) were only reduced for animals fed with 90% concentrate diet (4.00) compared to other diets (forage, 50, 60, 70 and 80%, 5.07 log10 genes copies). The decrease on protozoa numbers may be related to rumen pH, in which protozoa numbers drop 21% only when rumen pH was lower that 5.8 (80% concentrate in the diets). However, the methanogens numbers were higher in forage fed animals (4.40 log10 genes copies) that all grains diets (3.71 log10 genes copies). Thus, there was not a clear association between protozoa and methanogens numbers in the rumen, what has also been observed in other studies done recently using molecular tools. Although we did not have any data about methane emissions in this study, we could speculate the higher concentrate diets (more than 50% concentrate), there would be a lower methane emission, considering the low methanogens numbers in the rumen. MenosThe rumen is a very complex ecosystem in which there is a high diversity and concentration of rumen microbes. Animals, microbial and diets effects shape the rumen microbial population what make the rumen microbial manipulation a hard task to be done. There are many aspects about rumen microbial what the scientists and nutritionists would like to change, but the reduction in rumen methanogens and its activities is probably the first one. Considering the probable association between protozoa and rumen methanogens, this study aimed to evaluate their populations in the rumen of beef cattle during transition from a forage to high grains diets by qPCR. Four rumen fitted male Nellore Cattle were used, which were submit to diets from Brachiaria brizantha grass to high grains, from which rumen samples (solid and fluid) were collected when the diets were composed by zero (grazing animals), 50, 60, 70, 80 and 90% of concentrate on dry matter basis, as well as to get pH measurements. The DNA of rumen microbial population was isolated by using beads, phenol and chloroform, which makes possible to get high quantity and quality of DNA isolated. The DNA of rumen microbial population was amplified using groups primers (protozoa and methanogens) by qPCR method, which permits to calculate the genes copies numbers for every group. The rumen pH ranged from 6.51 to 5.73 for forage fed animals and for 90% concentrate fed animals, respectively. We found that protozoa numbers (log10 genes copies) we... Mostrar Tudo |
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Thesaurus NAL: |
Archaea; Methanogens; Rumen microorganisms. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/192971/1/2018-cpamt-bruno-pedreira-methanogens-rumen-protozoa-cattle-transition-grains-diets.pdf
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Marc: |
LEADER 03328nam a2200241 a 4500
001 2106183
005 2019-02-18
008 2018 bl uuuu u00u1 u #d
100 1 $aLIMA, L. R.
245 $aMethanogens and rumen protozoa in cattle during transition to high grain diets.$h[electronic resource]
260 $aIn: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 55.; CONGRESSO BRASILEIRO DE ZOOTECNIA, 28., 2018, Goiânia. Construindo saberes, formando pessoas e transformando a produção animal: anais. Viçosa: Sociedade Brasileira de Zootecnia; Brasília, DF: Associação Brasileira de Zootecnistas.$c2018
300 $anão paginado.
520 $aThe rumen is a very complex ecosystem in which there is a high diversity and concentration of rumen microbes. Animals, microbial and diets effects shape the rumen microbial population what make the rumen microbial manipulation a hard task to be done. There are many aspects about rumen microbial what the scientists and nutritionists would like to change, but the reduction in rumen methanogens and its activities is probably the first one. Considering the probable association between protozoa and rumen methanogens, this study aimed to evaluate their populations in the rumen of beef cattle during transition from a forage to high grains diets by qPCR. Four rumen fitted male Nellore Cattle were used, which were submit to diets from Brachiaria brizantha grass to high grains, from which rumen samples (solid and fluid) were collected when the diets were composed by zero (grazing animals), 50, 60, 70, 80 and 90% of concentrate on dry matter basis, as well as to get pH measurements. The DNA of rumen microbial population was isolated by using beads, phenol and chloroform, which makes possible to get high quantity and quality of DNA isolated. The DNA of rumen microbial population was amplified using groups primers (protozoa and methanogens) by qPCR method, which permits to calculate the genes copies numbers for every group. The rumen pH ranged from 6.51 to 5.73 for forage fed animals and for 90% concentrate fed animals, respectively. We found that protozoa numbers (log10 genes copies) were only reduced for animals fed with 90% concentrate diet (4.00) compared to other diets (forage, 50, 60, 70 and 80%, 5.07 log10 genes copies). The decrease on protozoa numbers may be related to rumen pH, in which protozoa numbers drop 21% only when rumen pH was lower that 5.8 (80% concentrate in the diets). However, the methanogens numbers were higher in forage fed animals (4.40 log10 genes copies) that all grains diets (3.71 log10 genes copies). Thus, there was not a clear association between protozoa and methanogens numbers in the rumen, what has also been observed in other studies done recently using molecular tools. Although we did not have any data about methane emissions in this study, we could speculate the higher concentrate diets (more than 50% concentrate), there would be a lower methane emission, considering the low methanogens numbers in the rumen.
650 $aArchaea
650 $aMethanogens
650 $aRumen microorganisms
700 1 $aMOMBACH, M. A.
700 1 $aPEDREIRA, B. C. e
700 1 $aFERREIRA, D. C.
700 1 $aCARVALHO, P. de
700 1 $aAMORIM, K. R. R. de
700 1 $aWEIMER, P. J.
700 1 $aCABRAL, L. da S.
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Embrapa Agrossilvipastoril (CPAMT) |
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